4/1/03

Introduction to Seed Development, Knock-Out Strategy, & Research
Lab Orientation and Tour
Pipetman Introduction and Accuracy
Fractionation of Gene-Specific PCR Products by Gel Electrophoresis

4/3/03

Introduction to PCR
Repeat Fractionation of Gene-Specific Products by Gel Electrophoresis
Setting up a Gene-Specific Polymerase Chain Reaction
Hands-on Introduction to Bioinformatics and Gene Annotation

4/8/03

Introduction to Reverse Genetics Knock-Out Strategy Using Arabidopsis
Hands-on Introduction to Goldberg Lab Webbook
DNA Gel Blot of Round One PCR Products Part One

4/10/03

DNA Gel Blot of Round One PCR Products Part Two
Introduction to Radiation and Use of Radioisotopes
Critical Analysis of Experimental Results

4/15/03

Pre-hybridization of DNA Gel Blots
Labeling of Gene-Specific Probes
Hybridization of DNA Gel Blots to Gene-Specific Probes

4/17/03

Washing DNA Gel Blots
Autoradiography

4/22/03

Critical Analysis of DNA Gel Blots & How to Confirm Results
PCR Amplification of Positive Gel Blot Bands From Relevant Superpools
Designing Primers for PCR , RT-PCR, Real-Time RT-PCR

4/24/03

Introduction to Sanger DNA Sequencing & Analyzing Results
Gel Purification of Amplified DNA Bands
Sequencing DNA Bands
Send Off Results to Wisconsin Knock-Out Facility for Round Two

4/29/03

Introduction to RNA Isolation, Gene Expression, and RT-PCR
Isolate RNA from Scarlet Runner Bean Embryos and Organs
Gel Electrophoresis of Isolated RNAs

5/01/03

DNase Treatment of RNA
Determine Concentration of RNA

5/06/03

cDNA Synthesis and RT-PCR
Fractionation of RT-PCR Products Using Gel Electrophoresis

5/08/03

Introduction to Microarrays, Arabidopsis GeneChip, and GeneSpring
Hands-on Analysis of Gene-Specific Expression Using GeneSpring
Review of Goldberg Lab Webbook and Data Entry

5/13/03

Presentation and Critical Analysis of Data

5/15/03

Group 1: Fractionation of Round One PCR Products
DNA Gel Blot of Round One PCR Products
Group 2: Fractionation of Round Two PCR Products
Re-Amplification of Positive Round Two DNA Bands

5/20/03

Group 1: Pre-Hybridization of DNA Gel Blots
Labeling of Gene-Specific Probes
Hybridization of DNA

Group 2: Gel-Purification of Positive Round Two DNA Bands
Sequencing Round Two DNA Bands

5/22/03

Introduction to Microscopy
Microscopic Analysis of Embryo Development
Microscopic Analysis of Cell-Specific Transcription Using GFP

5/27/03

Group 1: Introduction to DNA Extraction
DNA Extraction of Positive Seed Pools
Gel Electrophoresis of Isolated DNAs

Group 2: Re-Amplification of Positive Gel Blot Bands

5/29/03

Group 1: DNA Extraction of Positive Seed Pools
Gel Electrophoresis of Isolated DNAs

Group 2: Gel Purification of Amplified DNA Bands
Sequencing DNA Bands

6/03/03

Preparation and Summary of All Data

6/05/03

Presentation of Research Results For the Quarter

4/21/03

Ken Feldmann

Arabidopsis as a Model Organism

5/5/03

Richard Hamilton

How to Start a Biotechnology Company

5/14/03(Wednesday)

Ling Fong Chung

Patenting Genes

5/28/03 (Wednesday)

Greg Stock

Research Ethics/Human Genome

6/2/03

Bob Goldberg


Class

Natural Obsessions -- Reality?
How to Give a Scientific Lecture/Talk

What Have We Learned?