4/6/04

Introduction to Research & Goals For Quarter
Introduction to Research Blue Books and Goldberg Lab Webbook
Lab Orientation and Tour
Pipetman Introduction and Accuracy Experiments
Fractionation of Gene-Specific PCR Products by Gel Electrophoresis
Bioinformatics and Gene Annotation – Part I

4/8/04

Introduction to the Polymerase Chain Reaction (PCR)
Setting up a Gene-Specific Polymerase Chain Reaction
Fractionation of Gene-Specific Products by Gel Electrophoresis (Repeat)
Bioinformatics and Gene Annotation – Part II
Using the Goldberg Lab Webbook              

4/13/04

Introduction to DNA Gel Blots, Probes, and Hybridization -- Concepts
DNA Gel Blot of Madison Round One PCR Products Set #1

4/14/04

Disassemble DNA Blot Apparatus

Introduction to Radiation and Use of Radioisotopes
DNA Gel Blot of Madison Round One PCR Products Set #2
Critical Analysis of Experimental Results

4/20/04

Introduction to DNA Gel Blots, Probes, and Hybridization -- Techniques
Pre-hybridization of DNA Blots
Labeling of Gene-Specific Probes
Hybridization of DNA Blots with Gene-Specific Probes  

4/22/04

Washing DNA Blots
Autoradiography          

Developing Autoradiogram

4/27/04

Introduction to Madison and SALK T-DNA lines
How to Analyze DNA Blots & Confirm Results?
PCR Amplification of Positive Gel Blot Bands From Relevant Superpools
Sowing Seeds from Wild-Type (Ecotype Col-0) and SALK Lines

4/29/04

Introduction to Sanger DNA Sequencing & Analyzing Results (ESTDB)
Gel Purification of Amplified DNA Bands
Sequencing DNA Bands

5/04/04

Introduction to RNA Gene Expression, RNA extraction, and RT-PCR
Isolating RNA from Scarlet Runner Bean Embryos and Organs
Gel Electrophoresis of Isolated RNAs
Send Off Results to Wisconsin Knock-Out Facility for Round Two

5/06/04

DNase Treatment of RNA
Determining Concentration of RNA
Gel Electrophoresis of DNase Treated RNA

5/11/04

Introduction to Microarrays, Arabidopsis GeneChip‚, and GeneSpring
cDNA Synthesis and RT-PCR
Review of Goldberg Lab Webbook and Data Entry

5/13/04

Fractionation of RT-PCR Products Using Gel Electrophoresis
Oral Presentation of Results to Date (~15 min/student)

5/18/04

5/20/04

Fractionation of Madison Round Two PCR Products
Re-Amplification of Madison Positive Round Two DNA Bands
Identifying SALK Plants Containing T-DNA Insertion Using PCR – Part I

5/25/04

5/27/04

6/01/04

How to Phenotype Arabidopsis Plants and Mutant Seeds
Identifying SALK Plants Containing T-DNA Insertion Using PCR -- Part II
Determining the Phenotype of raspberry3 Mutant Plant Controls

6/03/04

Genotyping T-DNA-Tagged SALK Plants Using PCR – Part II
Determining the Phenotype T-DNA-Tagged SALK Plants

6/08/04

Ken Kessler

Radiation Training Course

4/12/04

Bob Goldberg

Using Genomics to Dissect Seed Development

4/19/04

Brandon Le

Using Reverse Genetics and Microarrays to
Understand Seed Gene Function

Diane Jofuku

Arabidopsis as a Model Plant

5/3/04

Chilton T-DNA & Arabidopsis KO Articles  

Mid-Quarter Research Techniques Review

Sixty Years of Exciting Research at UCLA

Research Ethics- Specifiv Case Studies