Gene Discovery Lab Protocols
Experiment ONE - Introduciton to General Molecular Biology Techniques
Experiment TWO - Shotgun Sequencing Using Massively Parallel Pyrosequencing (454) Technology
Experiment THREE - Screening SALK T-DNA Mutagenesis Lines (GENE ONE)
Experiment FOUR - RNA Isolation and RT-PCR Analysis of GENE ONE
Experiment FIVE - Amplification and Cloning an Upstream Region
Experiment SIX - Observation and Characterization of Known and Unknown Mutants
Experiment SEVEN - Screening SALK T-DNA Mutagenesis Lines (GENE TWO)
Experiment EIGHT - RNA Isolation and RT-PCR Analysis (GENE TWO)
Experiment NINE - Observation and Characterization of Known and Unknown Mutants
Appendix1 - Molecular Techniques
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